#27 - Evaluating the limitations of heterologous protein synthesis using genomic-scale modelling and multi-omics data in S. cerevisiae

Deepti Vipin (University of Hamburg)

Tuesday, 01 Dec 21:15 - 22:00 CET

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Abstract

Title: Evaluating the limitations of heterologous protein synthesis using genomic-scale modelling and multi-omics data in S. cerevisiae

Author(s): Deepti Vipin^a, Thomas E. Gorochowski^b and Zoya Ignatova^a

Affiliation(s): ^aInstitute for Biochemistry and Molecular Biology, University of Hamburg, Hamburg; ^bSchool of Biological Sciences, University of Bristol, Bristol

Abstract: A major challenge when optimizing the production of synthetic circuits is minimizing the burden they impose on the shared resources of a host cell. In this work, we employ genome-scale modeling to capture core biosynthetic processes and use these
to explain the ramifications of protein production from synthetic genetic circuits in the model host organism S. cerevisiae. By integrating experimental data from RNA sequencing, proteomics and tRNA microarrays, our model is able to identify limits in the protein production that can be achieved. Our work provides a simplified, comprehensive computational approach to examine the effect of cellular resource limitation in S. cerevisiae expressing different synthetic circuits.