#02 - Controlling synthetic cells and tissues

Michael Booth (University of Oxford)

Monday, 30 Nov 21:15 - 22:00 CET

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Contribution

Title: Controlling synthetic cells and tissues

Author(s): Michael Booth^a

Affiliations:^aUniversity of Oxford

Abstract: Synthetic cells and tissues, composed of lipid-bounded compartments with a minimal cellular functionality, might have applications in biotechnology, including studying cellular function and drug delivery. My group have developed methods to externally control the function of these soft biomaterials with multiple stimuli, including light, temperature, and chemical signals. A main focus of our work is on the generation and application of light-activated DNA (LA-DNA) to control cell-free expression systems within these compartments. In the future these synthetic cells and tissues may be used to study or repair living tissues.

References

[1] Hartmann, Denis, Jefferson M. Smith, Giacomo Mazzotti, Razia Chowdhry, and Michael J. Booth. “Controlling Gene Expression with Light: A Multidisciplinary Endeavour.” Biochemical Society Transactions 48, no. 4 (August 28, 2020): 1645–59. https://doi.org/10.1042/BST20200014.

[2] Downs, Florence G., David J. Lunn, Michael J. Booth, Joshua B. Sauer, William J. Ramsay, R. George Klemperer, Craig J. Hawker, and Hagan Bayley. “Multi-Responsive Hydrogel Structures from Patterned Droplet Networks.” Nature Chemistry 12, no. 4 (April 2020): 363–71. https://doi.org/10.1038/s41557-020-0444-1.

[3] Booth, Michael J., Idil Cazimoglu, and Hagan Bayley. “Controlled Deprotection and Release of a Small Molecule from a Compartmented Synthetic Tissue Module.” Communications Chemistry 2, no. 1 (December 12, 2019): 1–8. https://doi.org/10.1038/s42004-019-0244-y.

[4] Booth, Michael J., Vanessa Restrepo Schild, Stuart J. Box, and Hagan Bayley. “Light-Patterning of Synthetic Tissues with Single Droplet Resolution.” Scientific Reports 7, no. 1 (August 24, 2017): 9315. https://doi.org/10.1038/s41598-017-09394-9.

[5] Booth, Michael J., Vanessa Restrepo Schild, Alexander D. Graham, Sam N. Olof, and Hagan Bayley. “Light-Activated Communication in Synthetic Tissues.” Science Advances 2, no. 4 (April 1, 2016): e1600056. https://doi.org/10.1126/sciadv.1600056.